Targeting the hemangioblast with a novel cell type-specific enhancer
© Teixeira et al; licensee BioMed Central Ltd. 2011
Received: 22 July 2011
Accepted: 28 December 2011
Published: 28 December 2011
Hemangioblasts are known as the common precursors for primitive hematopoietic and endothelial lineages. Their existence has been supported mainly by the observation that both cell types develop in close proximity and by in vitro differentiation and genetic studies. However, more compelling evidence will arise from tracking their cell fates using a lineage-specific marker.
We report the identification of a hemangioblast-specific enhancer (Hb) located in the cis-regulatory region of chick Cerberus gene (cCer) that is able to direct the expression of enhanced green fluorescent protein (eGFP) to the precursors of yolk sac blood and endothelial cells in electroporated chick embryos. Moreover, we present the Hb-eGFP reporter as a powerful live imaging tool for visualizing hemangioblast cell fate and blood island morphogenesis.
We hereby introduce the Hb enhancer as a valuable resource for genetically targeting the hemangioblast population as well as for studying the dynamics of vascular and blood cell development.
In the early vertebrate embryo, both hematopoietic and endothelial lineages derive from aggregates of mesodermal cells that form the blood islands in the extraembryonic yolk sac . This observation led to the hypothesis that both lineages derive from a common precursor named the hemangioblast . Although still debatable, the existence of hemangioblasts is mainly supported by in vitro differentiation studies [3, 4] as well as by evidence that blood and endothelial progenitors express a number of genes in common, such as VEGFR2, GATA2, Lmo2 and Scl/Tal1 , some of which regulate the differentiation of both cell lineages [6–8]. Other than these in vitro and genetic studies, further insight into hemangioblast cell fate will require time-lapse imaging studies using a lineage-specific marker.
Hemangioblast reporters have been described in transgenic mouse and zebrafish models [9, 10]. However, a particularly suitable system for visualizing live hematovascular development is the yolk sac of the avian embryo [11, 12]. During the study of chick Cerberus (cCer) transcriptional regulation , we isolated a cis-regulatory region that drives reporter gene expression specifically in blood-island progenitors or hemangioblasts. Here, we report the identification and characterization of this novel hemangioblast-specific enhancer and reveal its potential as a live imaging tool for studying blood and vascular development.
Results and Discussion
Sequence analysis of the Hb enhancer identified binding sites for transcription factors implicated in endothelial and blood cell differentiation, namely ETS, GATA and FoxH1 [15–17] (Figure 1A). To determine which of these binding elements may be responsible for the regulation of hemangioblast-specific expression, we analyzed the expression of reporter constructs containing mutations in the ETS (E), GATA (G1 and G2) or FoxH1 (F) sites in the PCR8 sequence [17–19] (Figure 1A). Hemangioblast expression was unaltered in embryos electroporated with the Emut, G1mut and Fmut constructs, but abolished in those electroporated with the G2mut construct (Figure 1B). These observations demonstrate that the G2 site is essential for the induction or maintenance of transcription in hemangioblasts, and suggest that the GATA2 is a transcriptional activator of the Hb enhancer in blood-island progenitors .
In the avian embryo, hemangioblasts ingress through the posterior primitive streak between stages HH2 and HH9 . To characterize the expression pattern of the Hb-eGFP reporter, embryos were electroporated at either early or late stages (HH3-4 and HH5-6, respectively), placed in culture and observed at successive time points (HH4-13). In embryos electroporated at HH3-4, Hb-eGFP fluorescence was initially detected in posterior primitive streak cells (data not shown) and in a cVEGFR2-positive population of posterior extraembryonic cells (HH5; Figures 2A and 2B). At later stages, eGFP-positive cells migrate away from the embryo and form aggregates in the extraembryonic region (HH7; Figure 2C) that will give rise to the blood islands (HH9; Figure 2D). At HH11, Hb-eGFP fluorescence was restricted to differentiated blood islands and the vascular cells that connect them (Figure 2E and 2F). In embryos electroporated at HH5-6, Hb-eGFP expression was specifically detected in the blood islands that form closer to the embryo (Additional file 1). Taken together, these observations suggest that the Hb enhancer is continually activated in ingressing hemangioblasts that populate the extraembryonic region in a lateral to medial temporal progression .
To confirm the specificity of the Hb-eGFP reporter, we analyzed the gene expression profile of early Hb-eGFP-positive cells and investigated the co-localization of eGFP fluorescence with known markers of hemangioblast-derived cells. As expected, genes expressed in hemangioblasts, such as Lmo2 (+3.73 fold), Tal1 (+3.62 fold) and CD34 (+2.39 fold), are enriched in the Hb-eGFP-positive population, whereas those expressed in other cell types, such as the endoderm (e.g., Sox17), neuroectoderm (e.g., Otx2) and paraxial mesoderm (e.g., Msgn1), are downregulated (Figure 2G; Additional file 2). At a later stage (HH11), Hb-eGFP fluorescence is detected both in endothelial cells, which express cVEGFR2 protein (Figure 2H), and in blood cells, which express the RBC antigen (Figure 2I). However, Hb-eGFP expression is not observed in the SMA-positive smooth muscle cells that surround the blood islands (Figure 2J). This observation supports the hypothesis that smooth muscle cells are not derived from hemangioblasts .
Taken together, our observations indicate that Hb-eGFP expression is initiated in hemangioblasts as they emerge from the primitive streak and is detected in differentiated endothelial and blood cells at least until HH13 (data not shown). This pattern was confirmed by time-lapse imaging of live electroporated embryos. At low amplification, we could see the eGFP-fluorescent cells moving away from the posterior primitive streak, aggregating to form the blood islands, and giving rise to the vascular plexus by connecting the separate islands (Additional file 3). At higher magnification, we were able to follow the movements of individual endothelial and blood cells (Additional file 4), such as the interchange of cells between different blood islands and the cell division of an erythroblast. Moreover, in older embryos, the Hb-eGFP reporter proved to be a very useful tool to record blood cell flow in the vascular plexus of the yolk sac (Additional file 5).
In summary, we identified a hemangioblast enhancer located in cCer cis-regulatory region and describe its activity in developing chick embryos. Furthermore, we have used the Hb-eGFP reporter to characterize the gene expression profile of hemangioblasts and visualize blood island morphogenesis and differentiation in living embryos. In the future, the Hb-eGFP reporter may become a valuable genetic tool for targeting ectopic gene expression to the hemangioblast population as well as for studying live vasculogenesis and blood flow.
The isolation and cloning of cCer 5' genomic sequences was performed as previously reported . In particular, cCer regulatory sequences were amplified by PCR using the Cer0.36-eGFP construct DNA as template (primer sequences provided upon request), and sub-cloned into the SacI/SpeI restriction sites of the p1229-eGFP enhancerless vector, which carries the human beta-globin minimal promoter  upstream of the eGFP coding sequence (Clontech). Putative binding elements for ETS, GATA (sites 1 and 2) and FoxH1 transcription factors were identified in the analysis of cCer genomic sequences using MatInspector Professional release 7.4  and MatchTM  http://www.gene-regulation.com/. Mutations in these elements were designed according to the literature [18, 19, 26] and introduced into the PCR8 construct by PCR-based site-directed mutagenesis. The pCAGGS-RFP vector (gift from D. Henrique), which contains the CAGGS promoter and the cDNA of monomeric red fluorescent protein (RFP; Clontech)  was used to control the electroporation efficiency.
Embryo electroporation and imaging
Chicken embryos were explanted and electroporated at stages HH3-5  as described previously . Electroporated embryos were grown in New culture  until stages HH6-11, observed under a Zeiss SteREO Lumar fluorescence stereomicroscope (Carl Zeiss) and photographed using a Hamamatsu C8484 digital camera (Hamamatsu Photonics) and AxioVision software (Carl Zeiss).
Fluorescence-activated cell sorting (FACS)
Chicken embryos were electroporated at HH3 with Hb-eGFP and pCAGGS-RFP constructs, harvested at stage HH5-6 into three groups of four embryos each, dissociated into single cell suspensions using trypsin (Sigma-Aldrich) and filtered through a 35 μm cell strainer (BD Bioscience). The eGFP+ and eGFP-/RFP+ cell populations were FACS-sorted in a Moflo high-speed cell sorter (Beckman Coulter), using a 70 μm ceramic nozzle with 0.414MPa (60 psi) sheath pressure, a 488 nm laser line from a Coherent Sapphire 488-200 CDRH laser for eGFP excitation, and a 561 nm laser line from a CrystaLaser GCL-050-561 50 mW DPSS laser coupled to fiber optics (38 mW output) to excite RFP. eGFP+ and RFP+ cells were detected using 530/40 nm and 630/75 nm HQ band pass filters, respectively, and collected simultaneously into two different tubes containing RNAlater (Ambion).
RNA Isolation and Microarray Expression Analysis
Total RNA was extracted from triplicates of each cell population using the RNeasy Mini Kit (Qiagen). Concentration and purity was determined by spectrophotometry and integrity confirmed using an Agilent 2100 Bioanalyzer with a RNA 6000 Nano Assay (Agilent Technologies). Prior to processing for microarray hybridization, RNA samples from FACS-sorted eGFP+ and eGFP-/RFP+ populations were analyzed for the expression of control and marker genes (GAPDH, eGFP, RFP, cVEGFR2, cLmo2 and cBra) by reverse transcription polymerase chain reaction (data not shown). For each of the six samples, 40 ng of total RNA was processed according to the manufacturer's Two-Cycle Target Labeling Assay. Size distribution of the cRNA was assessed using an Agilent 2100 Bioanalyzer with a RNA 6000 Nano Assay. Affymetrix GeneChip Chicken Genome Arrays were hybridized at 45°C for 16 h with 15 μg of fragmented cRNA, washed and double-stained on an Affymetrix GeneChip Fluidics Station 450, and scanned on an Affymetrix GeneChip scanner 3000 7G. The arrays were analyzed using Affymetrix GCOS 1.4 and dChip 2008 software (http://www.dchip.org, Wong Lab, Harvard). Normalized CEL intensities of the six arrays were used to obtain gene expression indices based on a Perfect Match-only model. Only genes with a lower 90% confidence bound of the fold change above 1.7 were regard as differentially expressed and used for further analysis. Annotations for the 33.457 transcripts that are represented on the GeneChip Chicken Genome Array were obtained from the NetAffx database http://www.affymetrix.com as of September 2008. Out of this total number of transcripts, 658 were differentially expressed in the two populations, including 476 known genes (fold change > 1.7). Of these, 170 were upregulated and 306 downregulated in Hb-eGFP+ cells. Microarray data have been deposited in NCBI's Gene Expression Omnibus (GEO) with the accession number GSE32494.
In situ hybridization and Immunohistochemistry
Whole-mount in situ hybridization was performed as described previously . The cVegfr2 riboprobe fragment (nucleotides 2488-2771) was generated by RT-PCR and cloned into pGEMTeasy vector (Promega). Embryo images were acquired using a Leica MZ FLIII stereomicroscope, a Leica DFC420C digital camera (Leica Microsystems) and IrfanView software http://www.irfanview.com/.
For immunohistochemistry, electroporated embryos were fixed in 4% paraformaldehyde, cryoprotected in 15% sucrose, embedded in 7.5% gelatine/15% sucrose and cryosectioned at 16 or 20 μm. Immunostaining was performed using primary antibodies against avian VEGFR2 (gift from Anne Eichmann) , chicken Red Blood Cells (RBC; #103-4139; Rockland Immunochemicals), and alpha smooth muscle actin (SMA; #ab5694; Abcam), and secondary antibodies labeled with the fluorescent probe Alexa Fluor 568 (#A11004; Molecular Probes/Invitrogen) or Alexa Fluor 647 (#A21245; Molecular Probes). Cell nuclei were labeled with 4', 6-diamidino-2-phenylindole (DAPI; Molecular Probes). Sections were mounted in Fluorescence Mounting Medium (Dako), photographed using either a Leica DMRA2 fluorescence microscope with a HC PL Fluotar 20/0.50 objective (Leica Microsystems), Photometrics CoolSNAP HQ camera (Photometrics) and MetaMorph software (Molecular Devices; Figures 2F, Additional file 1B and 1C), or a Leica TCS SP5 confocal microscope with a HCX PL Apo CS 40/1.40-0.60 objective and Leica Application Suite software (Leica Microsystems; Figures 2H-J). Images were processed and assembled using Photoshop CS3 (Adobe Systems) and Imaris (Bitplane).
The authors thank Anne Eichmann for the VEGFR2 antibody, Domingos Henrique for the pCAGGS-RFP construct, Jörg Becker, Gabriel Martins, Nuno Moreno, Sofia Andrade and Paulo Duarte for technical assistance, and Jennifer Rowland for revising the manuscript. This work was supported by Centro de Biologia do Desenvolvimento and by Fundação para a Ciência e a Tecnologia (PPCDT/SAU-MMO/59725/2004).
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