Retinoic acid is a key regulatory switch determining the difference between lung and thyroid fates in Xenopus laevis
© Wang et al; licensee BioMed Central Ltd. 2011
Received: 27 July 2011
Accepted: 20 December 2011
Published: 20 December 2011
The lung and thyroid are derived from the anterior endoderm. Retinoic acid and Fgf signalling are known to be essential for development of the lung in mouse but little is known on how the lung and thyroid are specified in Xenopus.
If either retinoic acid or Fgf signalling is inhibited, there is no differentiation of the lung as assayed by expression of sftpb. There is no change in expression of thyroid gland markers when retinoic acid signalling is blocked after gastrulation and when Fgf signalling is inhibited there is a short window of time where pax2 expression is inhibited but expression of other markers is unaffected. If exogenous retinoic acid is given to the embryo between embryonic stages 20 and 26, the presumptive thyroid expresses sftpb and sftpc, specific markers of lung differentiation and expression of key thyroid transcription factors is lost. When the presumptive thyroid is transplanted into the posterior embryo, it also expresses sftpb, although pax2 expression is not blocked.
After gastrulation, retinoic acid is required for lung but not thyroid differentiation in Xenopus while Fgf signalling is needed for lung but only for early expression of pax2 in the thyroid. Exposure to retinoic acid can cause the presumptive thyroid to switch to a lung developmental program.
The differentiation of specific organs from the endoderm is directly related to their position along the anteroposterior axis of the vertebrate embryo. After initial specification of the endoderm, wnt, nodal, bone morphogenetic protein and fibroblast growth factor (Fgf) signals quickly subdivide the gut into the foregut, midgut and hindgut . Expression of hhex, sox2 and foxa2 are associated with the foregut  whereas expression of sox17  and cdx  genes is eventually restricted to posterior endoderm. The foregut will give rise to the thyroid, thymus, parathyroid, lung, liver, pancreas, and stomach.
Although specific transcription factors are associated with each of these organ systems in later development, expression of key transcription factors at early stages is not restricted to a specific organ system. In the foregut, nkx2.1 (or ttf1) is required for development of both the lung and thyroid suggesting a close relationship between these tissues but each organ has a distinct set of transcription factors that are required for differentiation. The thyroid also expresses hhex, pax8, and foxe1 , although in Xenopus and zebrafish, pax2 is the Pax gene expressed in the thyroid [6, 7]. In addition to nkx2.1, the lung expresses foxa2 and foxp2 [8–10]. The interplay of many different signalling pathways is necessary for defining both of these organs. Fibroblast growth factor (Fgf) signalling has been shown to be essential for both thyroid  and lung although the signal amplitude and timing are important for determining the output from the Fgf signal .
Recently, in addition to Fgf signalling, it has been demonstrated that graded levels of retinoic acid (RA) are important in chick for defining the different regions along the anterior-posterior axis of the developing endoderm . The most anterior endoderm, that gives rise to the thyroid, forms with little or no RA although RA is necessary for other anterior endoderm derivatives including the lung [14, 15] and pancreas [16, 17]. In frog, aldh1a2 (raldh2), encoding the enzyme primarily responsible for the synthesis of RA, is expressed dynamically throughout development. After gastrulation when patterning of the gut is being established, expression is found in the lateral plate mesoderm overlying the anterior gut with highest levels on the dorsal side. There are discrete regions of expression in the head but on the ventral side, there is little expression anterior to the forming heart [18, 19]. This would predict that the forming lung should be exposed to RA but that more posterior endoderm would be exposed to lower levels of RA. The most anterior endoderm, giving rise to the thyroid, should also have limited exposure to RA. Later in development, expression of aldh1a2 is found in specific gut regions where it is required for the gut looping process .
In Xenopus, differentiation markers that can be used to study development of the lung have only recently been identified . In that study, surfactant protein B (sftpb) and surfactant protein C (sftpc) were shown to have high sequence conservation with other homologues and were surprisingly early markers of lung in Xenopus, being expressed as early as stage 39. Sftpb and sftpc encode important constituents of pulmonary surfactant, a material that regulates surface tension in the lung alveoli and are expressed solely in the lung . Although the role of thyroid hormone in Xenopus biology has been extensively studied , the development of the thyroid is not well characterized. Expression studies have demonstrated key early transcription factors, identified as essential in other organisms, likely play a role in thyroid development in Xenopus including nkx2.1 , pax genes [6, 25], foxe1, and foxe4 , but how the thyroid is specified in frog is not known.
The goal of this study was to test the hypothesis that the underlying mechanism for the development of the lung and thyroid in Xenopus is similar to other model organisms. In doing so, we demonstrate that the requirement for RA and Fgf signalling in lung differentiation is conserved in Xenopus. Furthermore, if exogenous retinoic acid is applied to the embryo after gastrulation, the thyroid primordium will express sftpb and sftpc. In addition, expression of early transcription factors that are normally expressed in the thyroid but not the lung, pax2, foxe4, and hhex are lost in presumptive thyroid in response to retinoic acid. These results further demonstrate that spatial control of specific signalling factors is essential for the patterning of the foregut endoderm and that retinoic acid signalling is a key element of that process.
Temporal expression of lung and thyroid markers
Retinoic acid and FGF are required for lung development in Xenopus
Exogenous retinoic acid causes the thyroid to express the lung developmental program
Presence of pax2 and sftpb expression in the presumptive thyroid exposed to different concentrations of RA
1 μM RA
0.1 μM RA
0.01 μM RA
Effect of RA on presence of sftpb, pax2 and foxe4 expression in specific organs
St. 14 DMSO
St. 14 RA
St. 26 DMSO
St. 26 RA
St. 30 DMSO
St. 30 RA
St. 32 DMSO
St. 32 RA
St. 34 DMSO
St. 34 RA
St. 36 DMSO
St. 36 RA
RA and FGF are required for lung development in Xenopus
Little is known about the signalling mechanisms required for lung development in Xenopus. A recent study has suggested that wnt and hedgehog signalling are needed for later lung development based on the expression patterns of shh, wnt7b, wnt5a and wif1 but their functional roles have yet to be elucidated. Here we show that both RA (Figure 2) and Fgf (Figure 3) signalling are necessary for differentiation of the lung in Xenopus. Both of these signalling systems are required for lung differentiation in the mouse and the two systems have been linked as loss of RA results in reduced levels of Fgf10 and subsequent lung agenesis . It would appear that both RA and Fgf are very high in the hierarchy of lung specification as both are required well before the initial expression of nkx2.1.
The requirement for RA signalling is lost quite early in the development of the lung. If the antagonist is added as early as stage 20, well before the expression of nkx2.1, there is still detectable expression of sftpb. If the antagonist is added at stage 26, the expression of sftpb is essentially the same as control embryos. The sftpb promoter in humans has binding sites for both retinoic acid receptors and for nkx2.1 and the two transcription factors act synergistically to drive expression of sftpb . Although we cannot make any conclusions regarding the levels of sftpb expression using whole mount in situ hybridization, our results demonstrate that the activation of the retinoic acid receptors by RA is not a requirement for sftpb expression.
The requirement for Fgf also occurs early in Xenopus as blocking Fgf signalling at stage 20 causes a loss of nkx2.1 expression, although unlike RA, the requirement is maintained later as blocking at stage 26 still showed a complete loss of nkx2.1 (Figure 3) and subsequent sftpb expression (Additional File 1, Figure S1) in the lung.
Requirements of RA and Fgf signalling for development of the thyroid gland
When RA signalling was blocked, expression of thyroid markers was not affected (Figure 5). In mouse, Fgf8, regulated by tbx1 and arising from the secondary heart field, is required for normal thyroid development although loss of fgf8 did not eliminate the thyroid . In zebrafish, hand2 is upstream of fgf8 and this pathway is essential for thyroid development and again it is the cardiac mesoderm that is the source of the Fgf signal . Thus it is surprising that the SU5402 treatments had little effect on expression of either nkx2.1 or foxe4, although it was clear that SU5402 was able to cause developmental defects in the treated embryo, including loss of nkx2.1 expression in the lung, severe loss of tail (Figure 3) and loss of sprouty2 expression (Additional File 1, Figure S1).
We did find that the expression of pax2 in the thyroid was lost with early SU5402 treatments. The thyroid itself does not appear to be a direct target of the Fgf signal in zebrafish . If the potential intermediate tissue were already established before we treated with SU5402 in Xenopus, this could provide an explanation for the lack of effect on nkx2.1 and foxe4 expression. Identification of the intermediate tissue is needed in order to test this possibility. The lack of pax2 expression while nkx2.1 and foxe4 expression is maintained is perhaps expected. In pax8 knockout mice, the early thyroid primordium is maintained with normal nkx2.1 expression although the thyroid is eventually lost at later stages due to apoptosis [5, 33]. Thus, eventual loss of the thyroid might eventually be expected in Xenopus when Fgf signalling is blocked at very early stages.
RA causes presumptive thyroid tissue to express lung-specific markers
We have observed that addition of exogenous RA results in expression of sftpb and sftpc in the thyroid (Figure 2,4), both of which are clear markers of the differentiated lung. This does not seem to be simple ectopic expression as thyroid expression of hhex, pax2, and foxe4 are lost with the addition of RA (Figure 5,6). Hhex, pax8, foxe4, and nkx2.1 have been identified as forming a key transcription factor network for maintaining the thyroid  and it appears that a similar network exists in Xenopus. Pax2 substitutes for the expression of pax8  and based on our expression data, it appears that foxe4 is more closely related to the central thyroid region than foxe1 (Figure 5). Nevertheless, the early thyroid markers that we have examined are lost with the addition of RA suggesting that there has been a loss of the thyroid developmental program. The one exception is that expression of nkx2.1 is maintained but it is also normally expressed in the lung. The suppression of hhex expression by exogenous RA has also been observed in the chick although in that system there was also loss of nkx2.1 expression  suggesting that RA is sufficient to block thyroid development but not able to cause the fate switch that we observe in Xenopus. In the same chick study it was concluded that the thyroid must form in the absence or at very low levels of RA . Our results confirm this and suggest that in Xenopus, the absence of RA is actually required for thyroid development, as presence of RA would result in potential differentiation as lung tissue.
We were not able to look at any differentiation markers of thyroid. Although thyroid hormone has been extensively studied in terms of metamorphosis in Xenopus, little is known about the ontogeny of the enzymes required for thyroid hormone production. A study examining the expression of thyroid peroxidase, type II idothyronine deiodinase, and type III iodothyronine deiodinase showed that none of these were expressed in the thyroid before stage 41 . Interestingly, although initially described as thyroid gland expression, thyroid peroxidase is expressed in the lung at stage 43  further demonstrating the close relationship between these two organs. We also hoped to determine whether RA-treated thyroid progenitors would form structures reminiscent of a mature lung such as branching or alveoli. However, these are very late events in Xenopus development  and embryos treated with RA usually developed swelling around the heart that did not allow culturing to later stages.
The recent finding that sfrp5 and wnt11, both linked to the planar cell polarity pathway [36, 37], are necessary for foregut specification suggests that morphogenetic movements of the endoderm may be important for proper specification of the different organ systems . The requirement for morphogenesis suggests a relatively simple model whereby the early movements of the endoderm simply result in the furthest anterior endoderm, the presumptive thyroid, being beyond the mesodermal domains where retinoic acid is being synthesized. By simply physically moving the presumptive thyroid endoderm back to regions that would be predicted to have high levels of RA, the transplanted endoderm was able to express lung differentiation markers (Figure 7). However, we cannot rule out the possibility that other factors, present in the posterior embryo, might also be responsible for the transition.
Both RA and FGF signalling are required for the differentiation of the lung in Xenopus embryos. RA is required for lung development before the lung primordium expresses nkx2.1 and sftpb can still be expressed in embryos that are treated with an RA antagonist in later development. If the presumptive thyroid is exposed to RA before stage 20, early thyroid transcription factors are suppressed and lung differentiation markers will be expressed in the presumptive thyroid.
Female Xenopus laevis frogs were injected with 600-700 IU of human chorionic gonadotrophin (Sigma) to induce ovulation. In vitro fertilization of ovulated eggs was performed in 80% Steinberg's solution containing minced testis. Embryos were dejellied with 2.5% cysteine, pH 8.0, and cultured in 20% Steinberg's solution. Embryos were staged according to the Nieuwkoop and Faber staging table .
Cloning of sftpb and sftpc
Adult Xenopus laevis lung cDNA was used to amplify both sftpb and sftpc. The initial clones were amplified by polymerase chain reaction using the following sequences: sftpb forward 5'-cagtgggtccacaggatgac-3' and reverse 5'-gacggccccacactctttag-3' and sftpc forward 5'-ggctgcacatgagtcaaaaac-3" and reverse 5'-ctgttccggatccatttgtg-3'. The sequence obtained from the sftpb amplicon was used to generate new primers for a 5' rapid amplification of cDNA ends procedure that gave a longer cDNA clone that included the start codon. Sequencing of the clone showed that it matched previously identified sftpb sequence (Genbank Ref. NM_001096917.1). The sftpc sequence matched the previously identified sftpc sequence (Genbank Ref NM_001096721.1).
Embryos were treated with 1μM all-trans RA (Sigma) or 1μM pan RAR antagonist (Allergan #193109) [30, 39] in 20% Steinberg's solution at various stages depending on the experiment. Stock solutions for both RA and RAA were 1mM dissolved in DMSO, and therefore a control treatment was performed with 0.1% DMSO in 20% Steinberg's solution. Fgf signaling was inhibited by the addition of 10μM SU5402 (EMB Biosciences) in the presence of 100 μM ATP. Control treatments were DMSO and ATP alone.
Explants were removed at stage 18/20 using the cement gland as a guide. Explants were cut and maintained in 1 × MBS. Explants were transplanted by creating a small wound in the recipient embryo at the site for transplantation and the transplantation was also conducted in 1× MBS. After the explant had healed, the embryos were transferred back to 20% Steinberg's solution.
Whole-mount in situ hybridizations were performed according to  with modifications: embryos were blocked in Tris buffered saline, 20% heat treated sheep serum; and the Proteinase K, and RNase A steps were omitted. Antisense Dig-labelled riboprobes for sftpb and sftpc were generated by cutting the plasmids with Xba1 and transcribing with SP6 RNA polymerase according to established protocols . Antisense Dig-labelled riboprobes for pax2a , foxe1, foxe4, , nkx2.1 , and hhex  were also generated using the same methods. BM Purple (Roche Diagnostics) was used as the alkaline phosphatase substrate throughout and embryos were fixed for twenty minutes in MEMPFA before removing endogenous pigment in 1% hydrogen peroxide, 5% formamide, and 0.5%SSC for several hours after the colour reaction. For some images, the embryos were cleared by immersion in 1 part benzyl alcohol: 2 parts benzyl benzoate after dehydration in methanol. Embryos were visualized using a Leica MZ12 dissecting microscope and images were taken using Northern Eclipse software (Empix Imaging; Mississauga, ON, Canada).
We thank Dr. R. Chandraratna for providing the retinoic acid antagonist and advice on its use. We thank H. El-Hodori, A. Brandli, P. Krieg and A. Zorn for generously providing plasmids. Finally, we would like to thank anonymous reviewers for useful suggestions. This project was supported by grants awarded to TAD from the Canadian Institutes of Health Research (MOP-74663) and from the National Sciences and Engineering Research Council (Canada). SJD was supported by a CIHR doctoral fellowship. Initial seed funding for the project came from Lawson Health Research Institute Internal Research Fund program.
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