EMG1 is essential for mouse pre-implantation embryo development
© Wu et al; licensee BioMed Central Ltd. 2010
Received: 18 March 2010
Accepted: 21 September 2010
Published: 21 September 2010
Essential for mitotic growth 1 (EMG1) is a highly conserved nucleolar protein identified in yeast to have a critical function in ribosome biogenesis. A mutation in the human EMG1 homolog causes Bowen-Conradi syndrome (BCS), a developmental disorder characterized by severe growth failure and psychomotor retardation leading to death in early childhood. To begin to understand the role of EMG1 in mammalian development, and how its deficiency could lead to Bowen-Conradi syndrome, we have used mouse as a model. The expression of Emg1 during mouse development was examined and mice carrying a null mutation for Emg1 were generated and characterized.
Our studies indicated that Emg1 is broadly expressed during early mouse embryonic development. However, in late embryonic stages and during postnatal development, Emg1 exhibited specific expression patterns. To assess a developmental role for EMG1 in vivo, we exploited a mouse gene-targeting approach. Loss of EMG1 function in mice arrested embryonic development prior to the blastocyst stage. The arrested Emg1 -/- embryos exhibited defects in early cell lineage-specification as well as in nucleologenesis. Further, loss of p53, which has been shown to rescue some phenotypes resulting from defects in ribosome biogenesis, failed to rescue the Emg1 -/- pre-implantation lethality.
Our data demonstrate that Emg1 is highly expressed during mouse embryonic development, and essential for mouse pre-implantation development. The absolute requirement for EMG1 in early embryonic development is consistent with its essential role in yeast. Further, our findings also lend support to the previous study that showed Bowen-Conradi syndrome results from a partial EMG1 deficiency. A complete deficiency would not be expected to be compatible with a live birth.
Ribosome biogenesis is fundamental to cell growth and accounts for a substantial proportion of a cell's energy expenditure . The ribosomal RNAs (rRNAs) are central to the ribosome structure and function . The rRNA genes exist as tandem repeats and form the foci upon which the nucleoli form. The rRNA precursor (47S) is synthesized from the genes by RNA polymerase I and assembled with ribosomal proteins to form the 90S pre-ribosome. This 90S preribosome is matured to form the large-60S ribosomal subunit and the small-40S ribosomal subunit. The 60S subunit contains the 28S, 5.8S and 5S rRNAs as well as approximately 49 proteins, whereas the 40S subunit contains the 18S rRNA and approximately 33 proteins. It is estimated that 200 proteins are involved in assembling the mature ribosomes . Many of them have been studied in yeast, but not in mammals. Nonetheless, the proteins are highly conserved and as a starting point, it is reasonable to assume that they function similarly in mammals.
EMG1 (also known as Nep1) was initially identified as "Essential for Mitotic Growth" in yeast , and later was shown to be involved in the biogenesis of the mature 40S ribosome [5, 6]. Yeast EMG1 (yEMG1) is a 28 kDa protein primarily detected in the nucleolus [5, 6]. Because the deletion of yEMG1 in yeast is lethal, temperature sensitive mutations in this gene have been used to study the effects of its deficiency. Depletion of yEMG1 resulted in a reduction in 18S rRNA, a decrease in 40S ribosomal subunits and an increase in the ratio of 60S to 40S ribosomal subunits [5, 6]. These findings indicate an important role for EMG1 in the biogenesis of the 40S ribosome.
Deciphering the precise role of EMG1 in 40S ribosome biogenesis has been challenging. A temperature sensitive mutation in yEMG1 could be suppressed by the methyl donor S-adenosyl methionine (SAM)  or deletion of the snR57 gene encoding a snoRNA needed for 2'-O-ribose-methylation of G1570 in the 18S rRNA . Furthermore, yEMG1 was found to interact directly with snoRNA  and the 18S rRNA . Taken together, these findings suggested that yEMG1 functions to methylate the 18S rRNA, a concept that was later supported by the identification of yEMG1 as a SAM-dependent pseudouridine-N1-specific methyltransferase .
The EMG1 protein is highly conserved from archaebacteria to humans or mice . Expression of the human orthologue of EMG1 in yeast demonstrates that it is capable of suppressing the lethal defect in yEMG1 cells, indicating that EMG1 is both structurally and functionally conserved among these eukaryotes . More recently, a mutation in human EMG1, which significantly reduces EMG1 protein levels, has been found to cause Bowen-Conradi syndrome (BCS), an autosomal recessive disorder characterized by severely impaired prenatal and postnatal growth, profound psychomotor retardation, and death in early childhood . This finding strongly suggests that EMG1, as a key molecule in ribosomal synthesis, could be important for development. To get a better understanding of this, in the present study, we have attempted to generate an EMG1-deficient mouse and characterize the expression of Emg1 during mouse development. Our data demonstrates that EMG1 is essential for mouse pre-implantation development.
Results and Discussion
Expression of Emg1during mouse embryogenesis
To determine the expression pattern of Emg1 in post-implantation embryos, mouse embryos at E8.5-E15.5 were analyzed. At E8.5-E9.5, Emg1 was widely and strongly expressed and showed no clear tissue-specific pattern (Figure 2A-B). At later stages (E11.5-E15.5), however, Emg1 was found to be expressed at a low level in most embryonic tissues, but strongly in several regions, including the ventricular zone of the neuroepithelium (Figure 2C), the neural layer of retina (Figure 2D), the follicles of vibrissae (Figure 2E), thymus (Figure 2F), submandibular glands (Figure 2G), brown adipose tissue (Figure 2H), lung (Figure 2I), nephric tubules and renal mesenchyme (Figure 2J) and seminiferous tubules in the testis (not shown).
To examine the expression of Emg1 in extraembryonic tissue, we performed RNA in situ hybridization on E8.5-E9.5 mouse placenta. No Emg1 signal was detected in the trophoblast cells, while a control gene, Rtel (Regulator of Telomere length) showed strong expression in this cell lineage (Figure 2K) .
In adult mice, similar levels of Emg1 mRNA were detected in multiple tissues by Northern blot hybridization (Figure 2L), suggesting that EMG1 could be widely expressed during postnatal development. However, using RNA in situ hybridization assays, Emg1 cell-specific expression patterns were detected in several tissues (Figure 2M-Q). In the adult testis, Emg1 is highly and specifically expressed in both spermatogonia and early meiotic spermatocytes, but not in late stage spermatocytes (Figure 2M). A strong Emg1 signal was also identified in oocytes and the granulosa cells of the pre-antral follicles in the ovary (Figure 2N). In the adult mouse brain, Emg1 expression was mainly detected in the granular layer of neurons of the cerebellum (Figure 2O) as well as in the hippocampus (Figure 2P). Specific expression of Emg1 was also found in the crypts of the intestine (Figure 2Q).
Taken together, our gene expression data indicates that EMG1 is broadly distributed in the early developing embryos, but its expression is more restricted in the later stages of development. In adult mice, Emg1 also exhibits cell-specific expression, most notably in the gonads, brain and intestine. This expression pattern suggests that EMG1 may not only be important for early embryonic development, but could also be required for the development of several cell lineages at late developmental stages or during postnatal development.
Generation of the Emg1null mouse allele
To further study the developmental role of EMG1, we have mutated Emg1 in mice by homologous recombination. The mouse Emg1 gene contains 6 exons that encode a protein composed of 244 amino acids. In order to create an Emg1 null allele, a gene-targeting vector with a splice acceptor (SA)-IRES-βgeo-pA cassette was used to replace exons 2-6 and to remove approximately 80% of the Emg1 coding sequence (Figure 3A). Given that Emg1 is highly expressed in ES cells, the inserted SA-IRESβgeo cassette in the first intron of the Emg1 locus will trap exon 1 to turn on the expression of βgeo, a fusion protein of LacZ and neo. This was designed to allow us to significantly increase the targeting frequency, while also allowing us to establish a mouse allele in which a LacZ reporter is regulated by the endogenous Emg1 regulatory elements. Indeed, approximately 25% (11 out of 45) of the ES colonies obtained after G418 selection showed correct homologous recombination by Southern blot analysis using both 5' and 3' probes external to the targeting vector (Figure 3B-C). Furthermore, LacZ transgene expression in Emg +/- embryos was entirely consistent with the expression pattern established by in situ hybridization (Figure 1E and 3D). In this study, we have used two independently targeted ES lines to generate germline-transmitting chimeras that were bred with 129S1 or CD1 females to produce Emg1 null mutants for functional analysis.
The pre-implantation lethality of Emg1null mutants
Genotype analysis of progeny from Emg +/- intercrosses
No. of mice by genotyping
The E3.5 embryos from Emg1 +/- intercrosses, however, consistently showed a mixture of morula and blastocyst-stage embryos. Genotyping of these embryos demonstrated that whereas the blastocysts were either Emg1 +/+ or Emg1 +/- heterozygotes, the morula-stage embryos were Emg1 -/- mutants (Figure 4C). In Emg1 -/- morulae, the blastomeres flattened and tightly aligned themselves against each other to form a compact ball of cells. Immunofluoresence (IF) with anti-E-cadherin antibody revealed that Emg -/- morulae exhibited the same organized pattern of E-cadherin along the cell boundaries as the wild-type control (Figure 4D). These data indicate that Emg -/- embryos do reach the compaction stage of morula development.
To determine whether Emg1 -/- embryos at E3.5 were arrested at this stage or simply delayed, we further cultured these embryos in vitro. After 24 h of culture, while Emg1 +/+ and Emg1 +/- E3.5 embryos formed expanded blastocysts, none of the E3.5 Emg1 -/- embryos developed to blastocysts (Figure 4E). Instead, many cells in the cultured mutant embryos showed fragmented, pyknotic nuclei, suggestive of cell death. To determine whether cell death is indeed increased in these cultured mutant embryos, we performed a TUNEL assay. A significantly higher number of TUNEL-positive nuclei were detected in the mutant embryos than in Emg1 +/+ embryos (Figure 4F). In addition, very few cells in the cultured mutant embryos were positive in the BrdU labeling assay (Figure 4G). These data clearly indicate that Emg1 -/- embryos arrest prior to forming the blastocyst and subsequently the embryos undergo degeneration. Because of this severe phenotype, no Emg1-deficient cells could be established to perform further molecular investigations.
Specification of early cell lineages in Emg1 -/- embryos
Ribosomal biogenesis in Emg1 -/- mutants
EMG1 has been shown to be a highly conserved nucleolar protein required for ribosome biogenesis . Emg1-null mutants exhibit arrested development prior to the blastocyst stage, similar to that observed in other mouse models that lack factors involved in ribosomal RNA synthesis or processing, including RBM19 (RNA-binding motif protein 19) , pescadillo-1 (PES-1) , fibrillarin , RNA polymerase I or II , BYSL , SURF6  and RPS19 (ribosomal protein S19) . Some of these genetic mutations have been clearly demonstrated to cause severe defects in ribosomal biogenesis [19, 21, 22]. Thus, loss of EMG1 function in mice could also disrupt this biological pathway, leading to pre-implantation lethality.
To determine if Emg1 -/- embryos exhibit defective 40S ribosome biogenesis, similar to yeast depleted in yEMG1, we examined the level of mature 18S rRNA using reverse transcription (RT) followed by PCR. Although the levels of 18S rRNA are significantly reduced in yeast depleted in yEMG1, no detectable decrease in 18S rRNA were detected in Emg1 -/- morulae at E2.5 as compared to wild-type embryos at the same developmental stage (Figure 6B). Since E2.5 mouse morulae could contain residual maternal rRNAs, we used the same approach to analyze 18S rRNA in E3.5 Emg1 -/- embryos as compared to wild-type embryos with the same developmental stage. Again, no difference was observed (Figure 6C). Given that the levels of 18S rRNA in cells are very high, and small differences would not be detectable using this assay, we also looked for an increase in the precursors to the 18S rRNA, the pre-rRNA, but again no obvious difference between Emg1 -/- and wild type or heterozygous embryos was detected (Figure 6C). The unchanged expression of 18S rRNA and 47S rRNA in Emg1 -/- embryos was also indicated by RT followed by real-time PCR analysis (data not shown). Although this data differs from that in yeast, it is still possible that there is a delay in ribosomal RNA processing or assembly that was not detected using these assays. More sensitive approaches such as metabolic labeling or pre-rRNA specific probes may be required to show a delay in rRNA processing, as was recently shown in the study of a protein required for the maturation of the 60S ribosomal subunit in human cells . However, due to the early pre-implantation lethality of the Emg1 null allele, we are unable to derive EMG1-deficient cells in which to perform these assays. Future experiments with a conditional knockout of EMG1 will greatly help to address the role of EMG1 in the regulation of ribosomal biogenesis during development.
P53 deficiency does not rescue the pre-implantation arrest of Emg1 -/- mice
Previous studies have found that mutations in many proteins involved in ribosome biogenesis lead to an up-regulation of p53 [30–32], a key regulator of the cell cycle and apoptosis. The importance of p53 in the regulation of ribosome biogenesis has been addressed by studies showing that inhibition of p53 can suppress the effects of some defects in ribosome biogenesis. In mice, p53 inhibition was found to suppress the effects of mutation of Tcof (Treacher Collins syndrome-causing gene)  and Rps24 (Diamond-Blackfan anemia-causing gene) . These findings suggest that the inhibition of p53 may suppress the detrimental effects of mutations in other disorders of ribosomal biogenesis, such as EMG1 deficiency. To test this, E3.5 embryos were collected from intercrosses of Emg1/p53 double heterozygotes (Emg1 +/- /p53 +/- ) or Emg1 +/- /p53 -/- . A total of 5 Emg1 -/- /p53 -/- E3.5 embryos were identified, and all of them were found to be arrested at morula stage like the Emg -/- null mutants (Additional file 2). In addition, none of E3.5 Emg1 -/- /p53 -/- embryos developed to blastocysts during in vitro culture. Taken together, these data demonstrate that p53 inactivation fails to rescue the pre-implantation arrest of the Emg1 null allele.
In summary, we have demonstrated that EMG1 is essential for mouse pre-implantation. We showed that loss of EMG1 function specifically arrests early embryonic development at the morula stage, preventing blastocyst formation. This phenotype is consistent with our expression data showing that Emg1 is highly expressed during this critical developmental stage. However, due to the high expression of Emg1 in mouse oocytes (Figure 2N), which could be maternally transmitted into early developing embryos like other nucleolar components  (Figure 1D), we could not exclude the possibility that EMG1 is also required before the morula stage. Future experiments with a conditional knockout of EMG1 specifically in mouse oocytes will allow us to answer this question. Nevertheless, our study highlights a critical role of EMG1 in mouse early embryonic development.
The importance of EMG1 in development has also been demonstrated by our recent finding that this gene is mutated in human BCS syndrome, a severe developmental disorder with prenatal and postnatal growth retardation, profound psychomotor deficit, and death in early childhood . Because of this mutation, the EMG1 protein was found to be significantly reduced in fibroblasts of BCS patients . The residual protein that is detected is likely necessary to allow survival, as mice with a complete deficiency of EMG1 exhibit pre-implantation lethality. Therefore, the involvement of EMG1 in development could be dose-dependant. The hypomorphic mutation of EMG1 in BCS could specifically affect late embryonic development or certain cell lineages to cause BCS-associated phenotypes. In line with this, in this study, we found that Emg1 is predominately expressed in distinct cell types at late embryonic developmental stages or in adult (Figure 2). The unique expression of Emg1 in the granular neurons of cerebellum or in hippocampus could underly an important role for EMG1 in the control of psychomotor development, whose dysfunction is characteristic of BCS. Future experiments with mouse alleles to allow knockin of the BCS mutation, or a conditional allele, will allow us to address the pathological role(s) of EMG1 in vivo.
We have provided direct genetic evidence that EMG1 is essential for mouse pre-implantation. Given the requirement of yEMG1 in the biogenesis of the ribosomal 40S subunit, our study also highlights the critical role of ribosomal biogenesis in early development. The absolute requirement for EMG1 in mouse development is consistent with its essential role in yeast. Further, our findings also lend support to the previous study that showed Bowen-Conradi syndrome results from a partial EMG1 deficiency. A complete deficiency would not be expected to be compatible with a live birth.
Construction of the Emg1 gene-targeting vector
The Emg1 gene-targeting vector was made based on a PCR-based cloning strategy as described previously . Briefly, the mouse Emg1 genomic fragments required for the 5' and 3' arms of homology were PCR-amplified from the genomic DNA of R1 ES cells (on 129S1 background) with a high-fidelity polymerase (Clontech). After validation by DNA sequencing, the PCR products were cloned into two individual vectors that contain the SA-IRESβgeo cassette, and a pGKDTA fragment (the negative selection cassette), respectively. Subsequently, using the restriction enzymes and the cloning strategy as described , the DNA fragments were isolated, and assembled together to generate the gene-targeting vector.
Generation of Emg1 deficient mice
The Emg1 gene-targeting construct was linearized and electroporated into R1 ES cells, and then selected with G418 (250 μg/ml). The G418 resistant ES clones were screened by Southern blot analysis for the correctly targeted allele using EcoR1 (for the 5' external probe) and BamH1 (for the 3' external probe) digestion. Two independently targeted ES cell clones were used to generate chimeric mice that subsequently transmitted the genetic alternation through the germ line. The phenotypes of Emg1 -/- mutants derived from both targeted ES cell lines were indistinguishable. Mice were maintained on either 129S1 or on a mixed 129S1 and CD1/ICR background, in which Emg1 -/- developed the same phenotype. All mouse experiments were performed in accordance with procedures approved by the University of Manitoba Animal Care and Use Committee.
PCR and Southern blot analysis were applied for genotyping the Emg1 heterozygous mice. PCR was performed on ear-punched DNA. Primers to amplify the targeted allele were the sense primer (P1), located in intron 2 (5'-GTTCCTCAGCATATACGTGCT-3') and antisense primer (P2) specific for the SA-IRESβgeopA cassette (5'-GGGACAGGATAAGTATGACATCA-3'). To detect the wild-type Emg1 allele, P1 primer and an antisense primer (P3) locating in exon 2 (5'-TGTAAACCTGTAGCAAGCCAGCT-3') were used for PCR. Southern blot analysis was undertaken using standard protocols.
To genotype pre-implantation mouse embryos, a nested PCR method was applied. DNA was prepared by incubating E2.5-E3.5 embryos with 10 μl of Proteinase K buffer (10 mM Tris pH 8.3, 50 mM KCl, 2 mM MgCl2, 0.01% gelatin, 0.45% Nonidet P40, 0.45% Tween 20 and 500 μg/ml proteinase K) for 1 h at 55°C followed by incubation at 95°C for 10 min. 5 μl of DNA sample was then directly used for PCR using P1 and P2 (for the Emg1 targeted allele) and P1 and P3 (for the wild-type allele). 2 μl of products from the first round PCR were further PCR-amplified with the internal primers, generating 264 bp (wild-type allele) and 353 bp (targeted allele) products, respectively.
A similar approach was also applied to genotype the p53 null allele in Emg1/p53 double mutant pre-implantation embryos. The first round PCR was done using the primers as described previously . In the second round PCR, the following internal primers were utilized: 5'-TACCTCACTACAGGTGACCTG-3' (sense) and 5'-TCTTAGAGACAGTTGACTCCAG-3' (antisense) (for detecting the p53 wild-type allele), and 5'- TACCTCACTACAGGTGACCTG-3' (sense) and 5'-GTGATATTGCTGAAGAGCTTGG-3' (antisense) (for detecting the p53 null allele).
Early embryo isolation and in vitro culture
Emg1 +/- or Emg1 +/- /p53 +/- mice were intercrossed, and the females were examined for the presence of a vaginal plug which was set as embryonic day (0.5). Embryos at different stages of development (E2.5 through E3.5) were collected by either dissecting ampullae or flushing oviducts with M2 medium (Millipore). For in vitro culturing, E3.5 embryos were placed in KSOM-1/2AA medium (Millipore), and incubated at 37°C for 24 h.
Immuno-staining of pre-implantation embryos
Immuno-staining of pre-implantation embryos was performed based on the protocols provided by Dr. Janet Rossant http://www.sickkids.ca/research/rossant/custom/protocols.asp. The following antibodies were used: monoclonal anti-CDX2 (1:200, CDX2-88, BioGenex, CA, USA), monoclonal mouse anti-OCT3/4 (1:50, C10; Santa Cruz Biotechnology), rabbit anti-NANOG (1:200, Cosmo Bio), Rat anti-E-cadherin (1:100, Sigma), monoclonal mouse anti-B23 (1:50, Invitrogen) and rabbit anti-fibrillarin (1:100, Abcam). Secondary antibodies included Texas Red or Alexa488-conjugated goat anti-mouse, goat anti-rat and goat anti-rabbit (Molecular Probes). To visualize nuclei, embryos were stained with DAPI (0.5μg/ml) for 3 min at room temperature. Immuno-stained embryos were mounted onto microscopic slides with ProLong Gold (Invitrogen) and covered with glass cover slips. Images were collected using a Zeiss Axioplan 2 microscope to generate Z-stacks which were deconvolved using iterative algorithms program in Axio Vision 4.6.
For the BrdU labeling assay, E2.5-E3.5 embryos were incubated with KSOM-1/2AA medium containing 10 μM BrdU (Sigma) for 3 h, and were then fixed in 4% paraformaldehyde (PFA) for 10 min, permeabilized in 0.25% Triton X-100 for 10 min, treated with 2N HCl for 10 min, and detected with anti-BrdU antibody (Sigma).
E2.5-E3.5 mouse embryos were fixed in 4% PFA in PBS for 1 h at room temperature, and permeabilized for 1 h in PBS-0.5% Triton X-100. The embryos were then washed three times in PBS-0.1% Trion X-100 and incubated at 37°C for 1 h in a staining solution containing biotin-dUTP, terminal deoxynucleotide transferase (TdT), and detected using an ABC staining kit (Vector).
Whole mount x-gal staining
Pre-implantation embryos were fixed for 2 min with 1% PFA, 0.2% glutaraldehyde and 0.02% NP40 in PBS. After fixation, embryos were washed three times with PBS containing 0.02% NP40, and stained at 37°C overnight with a staining solution (4 mM K4Fe(CN)6, 4 mM K3Fe(CN)6, 2 mM MgCl2, and 0.2% X-gal in PBS). For E8.5-E9.5 embryos, embryos were fixed for 30 min with 4% PFA. After extensive washing with PBS containing 0.02% NP40 (three times, 20 min/each time), embryos were stained as described above.
RNA in situ hybridization
RNA in situ analysis of whole mount mouse embryos and frozen sections of mouse tissues were performed according to established protocols  with antisense and sense digoxigenin-labeled riboprobes which were in vitro transcribed from the full-length mouse Emg1 coding sequence. Mouse embryos were collected from pregnant outbred ICR female mice at E9.5-E15.5 days of gestation, and the adult tissues were harvested from two-month old ICR mice. All the samples were fixed in DEPC-treated 4% PFA at 4°C overnight.
RNA in situ hybridization on early mouse embryos (E2.5-E3.5) was performed essentially based on a described protocol . To preserve the pre-implantation embryos, the whole procedure was carried out in a transwell-insert (Corning).
Northern Blot Analysis
Total RNA from flash-frozen mouse tissues was extracted using TRIzol (Life Technologies, Inc.). 20 μg of total RNA was separated on a 1% agarose-formaldehyde gel and transferred to Hybond nylon membrane (Amersham). Hybridization was carried out in PerfectHyb (Sigma) with 1.5×106 cpm/ml probe which covers the whole coding sequence of Emg1.
RT-PCR analysis of pre-implantation embryos
Each blastocyst or morula was lysed with 5 μl ice-cold Cell Lysis II Buffer (Ambion) for 10 min at 75°C. 2 μl of lysate was used for PCR based genotyping, the rest was digested with DNase1 (0.08 unit/μl, Ambion) at 37°C for 15 min. After inactivation at 75°C for 5 min, 2 μl of DNase1-treated embryonic lysate was used in an RT reaction in a 10 μl volume using the OneStep RT-PCR kit (Qiagen) according to the manufacturer's instructions. The final concentration of specific primers (see the reverse primers described below) was 0.6 μM each. 2 μl RT mixture were then used for PCR reactions with the Multiplex PCR kit (Qiagen) and the primers described below. The PCR cycles contain an initial denaturation step of 95°C for 15 min and 40 cycles of 94°C for 30 s, 60°C for 90 s, and 72°C for 60 s, and a final 10 min extension step at 72°C. The PCR products were separated on a 12% polyacrylamide gel in 1× Tris-borate-EDTA buffer. The bands on the gel were visualized by the silver staining method as described .
The following primers were used for the above RT-PCR analysis: Mouse Emg1: forward (5'-TGAAGTGAACCCCCAGACTC-3') and reverse (5'-GAAGTGGTCGGACACTGGAT-3'). The amplified DNA band is 148 bp. Mouse pre-rRNA: forward (5'-CTCCTGTCTGTGGTGTCCAA-3') and reverse (5'-TGATACGGGCAGACACAGAA-3') in the 5' external transcribed spacer (5'-ETS) region of mouse 47S pre-rRNA . The size of the PCR product is 105 bp. Mouse 18S rRNA: forward (5'-GCAATTATTCCCCATGAACG-3') and reverse (5'-GGCCTCACTAAACCATCCAA-3'), which gives rise to a DNA band with 123 bp.
Essential for mitotic growth 1
inner cell mass
RNA-binding motif protein 19
ribosomal protein S19
Terminal deoxynucleotide transferase
We thank Dr. Janet Rossant at the University of Toronto and Dr. Hiroshi Hamada at Osaka University for providing reagents and advice on the characterization of mouse pre-implantation embryos. We also thank Mr. Biswajit Chowdhury for assistance with the mouse breeding and colony maintenance. This study was supported by funding from Genome Canada (grant number: 308-162340200-2000 to H.D.) and the Canadian Institute of Health Research (MOP 62786 to B.T.R.). S.S. is supported by a studentship from NSERC (Natural Sciences and Engineering Research Council of Canada), and H.D. is a recipient of Canada Research Chair.
- Warner JR: The economics of ribosome biosynthesis in yeast. Trends Biochem Sci. 1999, 24: 437-440. 10.1016/S0968-0004(99)01460-7.View ArticlePubMedGoogle Scholar
- Moss T, Langlois F, Gagnon-Kugler T, Stefanovsky V: A housekeeper with power of attorney: the rRNA genes in ribosome biogenesis. Cell Mol Life Sci. 2007, 64: 29-49. 10.1007/s00018-006-6278-1.View ArticlePubMedGoogle Scholar
- Connolly K, Culver G: Deconstructing ribosome construction. Trends Biochem Sci. 2009, 34: 256-263. 10.1016/j.tibs.2009.01.011.PubMed CentralView ArticlePubMedGoogle Scholar
- Hakuno F, Hughes DA, Yamamoto M: The Schizosaccharomyces pombe mra1 gene, which is required for cell growth and mating, can suppress the mating inefficiency caused by a deficit in the Ras1 activity. Genes Cells. 1996, 1: 303-315. 10.1046/j.1365-2443.1996.27029.x.View ArticlePubMedGoogle Scholar
- Liu PC, Thiele DJ: Novel stress-responsive genes EMG1 and NOP14 encode conserved, interacting proteins required for 40S ribosome biogenesis. Mol Biol Cell. 2001, 12: 3644-3657.PubMed CentralView ArticlePubMedGoogle Scholar
- Eschrich D, Buchhaupt M, Kotter P, Entian KD: Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in ribosome biogenesis. Curr Genet. 2002, 40: 326-338. 10.1007/s00294-001-0269-4.View ArticlePubMedGoogle Scholar
- Lowe TM, Eddy SR: A computational screen for methylation guide snoRNAs in yeast. Science. 1999, 283: 1168-1171. 10.1126/science.283.5405.1168.View ArticlePubMedGoogle Scholar
- Bernstein KA, Gallagher JE, Mitchell BM, Granneman S, Baserga SJ: The small-subunit processome is a ribosome assembly intermediate. Eukaryot Cell. 2004, 3: 1619-1626. 10.1128/EC.3.6.1619-1626.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Buchhaupt M, Meyer B, Kotter P, Entian KD: Genetic evidence for 18S rRNA binding and an Rps19p assembly function of yeast nucleolar protein Nep1p. Mol Genet Genomics. 2006, 276: 273-284. 10.1007/s00438-006-0132-x.View ArticlePubMedGoogle Scholar
- Wurm JP, Meyer B, Bahr U, Held M, Frolow O, Kotter P, Engels JW, Heckel A, Karas M, Entian KD, Wohnert J: The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase. Nucleic Acids Res. 2010Google Scholar
- Taylor AB, Meyer B, Leal BZ, Kotter P, Schirf V, Demeler B, Hart PJ, Entian KD, Wohnert J: The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site. Nucleic Acids Res. 2008, 36: 1542-1554. 10.1093/nar/gkm1172.PubMed CentralView ArticlePubMedGoogle Scholar
- Armistead J, Khatkar S, Meyer B, Mark BL, Patel N, Coghlan G, Lamont RE, Liu S, Wiechert J, Cattini PA, Koetter P, Wrogemann K, Greenberg CR, Entian KD, Zelinski T, Triggs-Raine B: Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome. Am J Hum Genet. 2009, 84: 728-739. 10.1016/j.ajhg.2009.04.017.PubMed CentralView ArticlePubMedGoogle Scholar
- Ding H, Schertzer M, Wu X, Gertsenstein M, Selig S, Kammori M, Pourvali R, Poon S, Vulto I, Chavez E, Tam PP, Nagy A, Lansdorp PM: Regulation of murine telomere length by Rtel: an essential gene encoding a helicase-like protein. Cell. 2004, 117: 873-886. 10.1016/j.cell.2004.05.026.View ArticlePubMedGoogle Scholar
- Palmieri SL, Peter W, Hess H, Scholer HR: Oct-4 transcription factor is differentially expressed in the mouse embryo during establishment of the first two extraembryonic cell lineages involved in implantation. Dev Biol. 1994, 166: 259-267. 10.1006/dbio.1994.1312.View ArticlePubMedGoogle Scholar
- Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S, Smith A: Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell. 2003, 113: 643-655. 10.1016/S0092-8674(03)00392-1.View ArticlePubMedGoogle Scholar
- Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S: The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 2003, 113: 631-642. 10.1016/S0092-8674(03)00393-3.View ArticlePubMedGoogle Scholar
- Beck F, Erler T, Russell A, James R: Expression of Cdx-2 in the mouse embryo and placenta: possible role in patterning of the extra-embryonic membranes. Dev Dyn. 1995, 204: 219-227.View ArticlePubMedGoogle Scholar
- Zhang J, Tomasini AJ, Mayer AN: RBM19 is essential for preimplantation development in the mouse. BMC Dev Biol. 2008, 8: 115-10.1186/1471-213X-8-115.PubMed CentralView ArticlePubMedGoogle Scholar
- Lerch-Gaggl A, Haque J, Li J, Ning G, Traktman P, Duncan SA: Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation. J Biol Chem. 2002, 277: 45347-45355. 10.1074/jbc.M208338200.View ArticlePubMedGoogle Scholar
- Newton K, Petfalski E, Tollervey D, Caceres JF: Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol. 2003, 23: 8519-8527. 10.1128/MCB.23.23.8519-8527.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Chen H, Li Z, Haruna K, Li Z, Li Z, Semba K, Araki M, Yamamura K, Araki K: Early pre-implantation lethality in mice carrying truncated mutation in the RNA polymerase 1-2 gene. Biochem Biophys Res Commun. 2008, 365: 636-642. 10.1016/j.bbrc.2007.11.019.View ArticlePubMedGoogle Scholar
- Adachi K, Soeta-Saneyoshi C, Sagara H, Iwakura Y: Crucial role of Bysl in mammalian preimplantation development as an integral factor for 40S ribosome biogenesis. Mol Cell Biol. 2007, 27: 2202-2214. 10.1128/MCB.01908-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Romanova LG, Anger M, Zatsepina OV, Schultz RM: Implication of nucleolar protein SURF6 in ribosome biogenesis and preimplantation mouse development. Biol Reprod. 2006, 75: 690-696. 10.1095/biolreprod.106.054072.View ArticlePubMedGoogle Scholar
- Matsson H, Davey EJ, Draptchinskaia N, Hamaguchi I, Ooka A, Leveen P, Forsberg E, Karisson S, Dahl N: Targeted disruption of the ribosomal protein S19 gene is lethal prior to implantation. Mol Cell Biol. 2004, 24: 4032-4037. 10.1128/MCB.24.9.4032-4037.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Geuskens M, Alexandre H: Ultrastructural and autoradiographic studies of nucleolar development and rDNA transcription in preimplantation mouse embryos. Cell Differ. 1984, 14: 125-134. 10.1016/0045-6039(84)90037-X.View ArticlePubMedGoogle Scholar
- Flechon JE, Kopecny V: The nature of the 'nucleolus precursor body' in early preimplantation embryos: a review of fine-structure cytochemical, immunocytochemical and autoradiographic data related to nucleolar function. Zygote. 1998, 6: 183-191. 10.1017/S0967199498000112.View ArticlePubMedGoogle Scholar
- Zatsepina O, Baly C, Chebrout M, Debey P: The step-wise assembly of a functional nucleolus in preimplantation mouse embryos involves the cajal (coiled) body. Dev Biol. 2003, 253: 66-83. 10.1006/dbio.2002.0865.View ArticlePubMedGoogle Scholar
- Flechon JE, Kopecny V: The nature of the 'nucleolus precursor body' in early preimplantation embryos: a review of fine-structure cytochemical, immunocytochemical and autoradiographic data related to nucleolar function. Zygote. 1998, 6: 183-191. 10.1017/S0967199498000112.View ArticlePubMedGoogle Scholar
- Castle CD, Cassimere EK, Lee J, Denicourt C: Las1L Is a Nucleolar Protein Required for Cell Proliferation and Ribosome Biogenesis. Mol Cell Biol. 2010, 30: 4404-4414. 10.1128/MCB.00358-10.PubMed CentralView ArticlePubMedGoogle Scholar
- Panic L, Montagne J, Cokaric M, Volarevic S: S6-haploinsufficiency activates the p53 tumor suppressor. Cell Cycle. 2007, 6: 20-24.View ArticlePubMedGoogle Scholar
- Pestov DG, Strezoska Z, Lau LF: Evidence of p53-dependent cross-talk between ribosome biogenesis and the cell cycle: effects of nucleolar protein Bop1 on G(1)/S transition. Mol Cell Biol. 2001, 21: 4246-4255. 10.1128/MCB.21.13.4246-4255.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Rubbi CP, Milner J: Disruption of the nucleolus mediates stabilization of p53 in response to DNA damage and other stresses. EMBO J. 2003, 22: 6068-6077. 10.1093/emboj/cdg579.PubMed CentralView ArticlePubMedGoogle Scholar
- Jones NC, Lynn ML, Gaudenz K, Sakai D, Aoto K, Rey JP, Glynn EF, Ellington L, Du C, Dixon J, Dixon MJ, Trainor PA: Prevention of the neurocristopathy Treacher Collins syndrome through inhibition of p53 function. Nat Med. 2008, 14: 125-133. 10.1038/nm1725.PubMed CentralView ArticlePubMedGoogle Scholar
- Barkic M, Crnomarkovic S, Grabusic K, Bogetic I, Panic L, Tamarut S, Cokaric M, Jeric I, Vidak S, Volarevic S: The p53 tumor suppressor causes congenital malformations in Rpl24-deficient mice and promotes their survival. Mol Cell Biol. 2009, 29: 2489-2504. 10.1128/MCB.01588-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Ogushi S, Palmieri C, Fulka H, Saitou M, Miyano T, Fulka J: The maternal nucleolus is essential for early embryonic development in mammals. Science. 2008, 319: 613-616. 10.1126/science.1151276.View ArticlePubMedGoogle Scholar
- Lowry RB, Innes AM, Bernier FP, McLeod DR, Greenberg CR, Chudley AE, Chodirker B, Marles SL, Crumley MJ, Loredo-Osti JC, Morgan K, Fujiwara TM: Bowen-Conradi syndrome: a clinical and genetic study. Am J Med Genet. 2003, 120A: 423-428. 10.1002/ajmg.a.20059.View ArticlePubMedGoogle Scholar
- Wu X, Ding H: Generation of conditional knockout alleles for PDGF-C. Genesis. 2007, 45: 653-657. 10.1002/dvg.20339.View ArticlePubMedGoogle Scholar
- Jacks T, Remington L, Williams BO, Schmitt EM, Halachmi S, Bronson RT, Weinberg RA: Tumor spectrum analysis in p53-mutant mice. Curr Biol. 1994, 4: 1-7. 10.1016/S0960-9822(00)00002-6.View ArticlePubMedGoogle Scholar
- Ding H, Wu X, Kim I, Tam PP, Koh GY, Nagy A: The mouse Pdgfc gene: dynamic expression in embryonic tissues during organogenesis. Mech Dev. 2000, 96: 209-213. 10.1016/S0925-4773(00)00425-1.View ArticlePubMedGoogle Scholar
- Piette D, Hendrickx M, Willems E, Kemp CR, Leyns L: An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies. Nat Protoc. 2008, 3: 1194-1201. 10.1038/nprot.2008.103.View ArticlePubMedGoogle Scholar
- Bassam BJ, Gresshoff PM: Silver staining DNA in polyacrylamide gels. Nat Protoc. 2007, 2: 2649-2654. 10.1038/nprot.2007.330.View ArticlePubMedGoogle Scholar
- Strezoska Z, Pestov DG, Lau LF: Functional inactivation of the mouse nucleolar protein Bop1 inhibits multiple steps in pre-rRNA processing and blocks cell cycle progression. J Biol Chem. 2002, 277: 29617-29625. 10.1074/jbc.M204381200.View ArticlePubMedGoogle Scholar
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